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Lecture 12. Microbial Ecology

1.  Objective

The objective is to introduce major roles of microorganisms in nature, including classification of microorganisms, aerobic and anaerobic metabolism, biogeochemical cycles, interactions of microorganisms with plants and animals, microorganisms in extreme environments, and biomethanogenesis.

2.  Classification of Microorganisms

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Viruses (no cellular structure, nucleic acid plus one or more proteins, lack metabolic machinery)

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Bacteria (procaryotic, regular bacteria, archeans, bluegreen bacteria)

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Protistans (eucaryotic, no tissue, often single celled; fungi, algae, protozoa)

3.  Aerobic Versus Anaerobic Organisms

3.1.  Aerobic Respiration (12-1)

3.2.  Anaerobic Respiration (12-2)

3.3.  Fermentation (12-3)

3.4.  Summary (12-4)

3.5.  Methanogenesis (12-5)

 

4.  Biogeochemical Cycles

4.1.  Carbon (12-6)

4.2.  Nitrogen (12-7)

4.3.  Sulfur (12-8)

4.4.  Phosphorus (12-9)

 

5.  Animal-Microorganism Interactions

5.1.  Symbiosis

bulletrumen (a) (12-10) (b) (12-11)
bullettermites (12-12)

5.2.  Pathogenic

bulletdiptheria (12-13)
bulletsyphilis (12-14)
bulletplague (12-15)

 

6.  Plant-Microorganism Interactions

6.1.  Symbiotic

bulletlegumes (Rhizobium) (12-16)

6.2.  Pathogenic

bulletplant virus infections (12-17)
bulletgall tumors on willow (12-18) (caused by plasmid gene transfer from Agrobacterium tumefaciens)

 

7.  Microbial Ecology of Extreme Environments

7.1.  High and Low Temperature (12-19)

bulletcyanobacteria in hot springs (12-20)

7.2.  pH Extremes

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leaching (12-21)

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acid mine drainage (12-22)

7.3.  Limited Substrate Concentration (12-23)

7.4.  High Salinity (12-24)

7.5.  Pressure Extremes (12-25)

8.  Ecology of Methanogenesis

bulletEM of wood digester (12-26)
bulletAnaerobic zones in a lake (12-27)
bulletFate of methane in lake ecosystem (12-28)
bulletExperimental data of methane production in lakes (12-29)
bulletExperimental data of methane production versus sedimentation in lakes (12-30)
bulletSampling sediment through ice (12-31)
bulletGas sampling cone (12-32)
bulletMethanogenesis in rumen (12-33) 
bulletMethane production by termites (12-34)
bulletMethane production from wetwood of trees (12-35) 
bulletGlobal warming potentials of greenhouse gases (12-36)

 

9.  Critical Concepts or Questions

9.1.  Define and give five examples of bacteria, bluegreen algae, protozoa, algae, and fungi.

9.2.  Define aerobic respiration, anaerobic respiration, and fermentation.   Give three examples and discuss the ecological role of each type of metabolism.

9.3.  Briefly outline five benefitial and five harmful interactions of microorganisms to plants.

9.4.  Briefly outline five benefitial and five harmful interactions of microorganisms to animals.

9.5.  Outline the involvement of microorganisms in the carbon, nitrogen, and phosphorus cycles.

9.6.  What are five exreme environments where bacteria live?  Where (be specific) would you go to isolate them.

9.7.  What is the role of methanogenic bacteria in nature?  What would be the ecological impact of a phage that kills all methanogens?

9.8.  How are methanogenic bacteria used by humans in industry?

10.  Vocabulary

archaebacterium bacterial flagellum bacteriophage
binary fission chemosynthetic autotroph rhizosphere
endospore endospore eubacterium
eyespot heterocyst heterotroph
microbe multicellularity protistan
photosynthetic autotroph xenobiotic symbiosis
virus aerobic anaerobic
facultative nitrification denitrification
nitrogen fixation Nitrosomonas Nitrobacter
legume in situ in vitro
in vivo Azotobacter inoculum
barophile halophile psychrophilic
hydrothermal vent Thiothrix Desulfovibrio
sulfur-oxidizing bacteria sulfur-reducing bacteria Rhizobium
humus methanogenesis volatile acids
pyrite oxidation microbial leaching micorrhizae

 

11.  Webpage Links

ENZYME BIOCHEMISTRY REVIEW - S. Crotty @ MIT [*SELECT*] Enzyme Biochemistry: Chemical energetics, enzyme mechanisms, Michaelis-Menten Kinetics, inhibition.

ABE4660 Applied Microbial Biotechnology - D. Chynoweth @ Univ of FL

Environmental Engineering Student Projects - Chemical Engineering at RPI Great variety of topics: From sedimentation to brewing to aeration to enzymes to fermentation, etc.

Saccharomyces Genomic Information Resource - Saccharomyces Genome Database & Stanford DNA Sequence & Technology Center Yeast Protein Project, World Wide. Links to much of the research activity.

 

12.  Illustration Documentation